The effect of vitamin E supplementation on brain tissue element levels in epileptic rats

L. Ozturk-Sonmez, E. Tutkun, E. Agar, M. Ayyildiz, R. Mogulkoc, A. K. Baltaci


The aim of this study was to investigate how the application of vitamin E affected the levels of chemical elements in the brain tissues of epilepsy-induced rats. The sample of 40 adult male rats was separated into 4 equal groups: Group 1: control, Group 2: vitamin E; Group 3: penicillin to promote epileptic form activity and Group 4: penicillin + vitamin E. After three months of treatment, an Atomic Absorption Spectrophotometer was used to analyze the presence of the elements in brain tissue sections (brain, brainstem, cerebellum) of the decapitated animals. The levels of magnesium in the groups that received vitamin E (G2 and 4) were significantly higher than in the control group (G1) and the first epilepsy group (G3) (p<.05).Chrome and zinc levels in brain, brainstem, and cerebellum tissue of the two epilepsy groups (G3–4) decreased significantly compared to the control group (G1) and the vitamin E group (G2) (p<.05). The levels of copper in the brainstem and lead in the cerebellum of the first epilepsy group (G3) were higher than in all other groups (p<.05). The findings showed that the application of vitamin E in experimental epilepsy may have a limited effect on element metabolism in brain tissue. A decline in zinc levels in the brain, brainstem and cerebellum tissues in epilepsy groups constitutes another result of our study. This should be examined further to determine whether decreased levels of zinc play a role in epilepsy pathogenesis.



Epilepsy, Penicillin, Vitamin E, Brain, Element metabolism, Rat

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Aygun H., Ayyildiz M., Agar E. Effects of vitamin D and paricalcitol on epileptogenesis and behavioral properties of WAG/Rij rats with absence epilepsy. Epilepsy. Res. 157: 106208, 2019.

Baltaci A.K., Mogulkoc R., Akil M., Bicer M. Review - Selenium - Its metabolism and relation to exercise. Pak. J. Pharm. Sci. 29: 1719-1725, 2016.

Baltaci A.K., Mogulkoc R., Baltaci S.B. (2019): Review: The role of zinc in the endocrine system. Pak. J. Pharm. Sci. 32:231-239, 2019.

Baltaci S.B., Mogulkoc R., Baltaci A.K. Molecular Mechanisms of Early and Late LTP. Neurochem. Res. 44:281-296, 2019.

Baraka A.M., Hassab El Nabi W., El Ghotni S. Investigating the role of zinc in a rat model of epilepsy. CNS. Neurosci. Ther. 18:327-333, 2012.

Barry-Sterman M., Shouse M.N., Fairchild M.D., Belsito O. (1986): Kindled seizure induction alters and is altered by zinc absorption. Brain Res. 383: 382-386, 1986.

Blumcke I., Coras R., Miyata H., Ozkara C. (2012): Defining clinico-neuropathological subtypes of mesial temporal lobe epilepsy with hippocampal sclerosis. Brain. Pathol. 22: 402–411, 2012.

Brodie M.J., Barry S.J., Bamagous G.A., Norrie J.D, Kwan P. (2012): Patterns of treatment response in newly diagnosed epilepsy. Neurology. 78: 1548-1554, 2012.

Cecchini T., Ciaroni S., Ferri P., Ambrogini P., Cuppini R., Santi S., Del Grande P. Alpha - tocopherol, an exogenous factor of adult hippocampal neurogenesis regulation. J. Neurosci. Res. 73:447-455, 2003.

Dedeurwaerdere S., Fang K., Chow M., Shen Y.T., Noordman I., vanRaay L., Faggian N., Porritt M., Egan G.F., O'Brien T.J. Manganese-enhanced MRI reflects seizure outcome in a model for mesial temporal lobe epilepsy. Neuroimage. 68: 30-38, 2012.

DiAngelantonio S., Murana E., Cocco S., Scala F., Bertollini C., Molinari M.G., Lauro C., Bregestovski P., Limatola C., Ragozzino D. A role for intracellular zinc in glioma alteration of neuronal chloride equilibrium. Cell. Death. Dis. 30: 5:e1501, 2014.

Doboszewska U., Młyniec K., Wlaź A., Poleszak E., Nowak G., Wlaź P. Zinc signaling and epilepsy. Pharmacol. Ther. 193:156-177, 2019.

Elsas S.M., Hazany S., Gregory W.L., Mody I. (2009): Hippocampal zinc infusion delays the development of after discharges and seizures in a kindling model of epilepsy. Epilepsia. 50: 870-879, 2009.

Erbayat Altay E., Serdaroglu A., Gucuyener K., Bilir E., Karabacak N.I., Thio L.L. Rotational vestibular epilepsy from the temporo-parieto-occipital junction. Neurology. 65: 1675-1676, 2005.

Fisher R.S. Animalmodels of epilepsies. Brain. Res. Rev. 14: 245-278, 1989.

Khan M.Z. A possible significant role of zinc and GPR39 zinc sensing receptor in Alzheimer disease and epilepsy. Biomed. Pharmacother. 79: 263-272, 2016.

Kiasalari Z., Khalili M., Shafiee S., Roghani M. The effect of Vitamin E on learning and memory deficits in intra hippocampal kainate-induced temporal lobe epilepsy in rats. Indian. J. Pharmacol. 48: 11-14, 2016.

Kim J.H., Jang B.G., Choi B.Y., Kwon L.M., Sohn M., Song H.K., Suh S.W. Zinc chelation reduces hippocampal neurogenesis after pilocarpine-induced seizure. PLoSOne. 7: e48543, 2012.

Kozan R., Ayyildiz M., Yildirim M., Agar E. The influence of ethanol intake and its withdrawal on the anticonvulsant effect of alpha-tocopherol in the penicillin-induced epileptiform activity in rats. Neurotoxicology. 28: 463-470, 2007.

Menon B., Ramalingam K., Kumar R.V. Low plasma antioxidant status in patients with epilepsy and the role of antiepileptic drugs on oxidative stress. Ann. Indian. Acad. Neurol. 17: 398-404, 2014.

Nakhaee Moghadam M., Teimouri A., Khajeh A., Hoseini S.B. Bone metabolism disorder in epileptic children. Iran. J. Child. Neurol. 12: 17-24, 2018.

Nazıroğlu M., Yurekli V.A. Effects of antiepileptic drugs on antioxidant and oxidant molecular pathways: focus on trace elements. Cell. Mol. Neurobiol. 33: 589-599, 2013.

Niki E. Role of vitamin E as a lipid-soluble peroxyl radical scavenger: in vitro and in vivo evidence. Free Radic. Biol. Med. 66: 3-12, 2014.

Osborn K.E., Shytle R.D., Frontera A.T., Soble J.R., Schoenberg M.R. Addressing potential role of magnesium dyshome ostasisto improve treatment efficacy for epilepsy: A reexamination of the literature. J. Clin. Pharmacol. 56: 260-265, 2016.

Owoeye O., Adedara I.A., Bakare O.S., Adeyemo O.A., Egun C., Farombi E.O. Kolaviron and vitamin E ameliorate hematotoxicity and oxidative stress in brains of prepubertal rats treated with an anticonvulsant phenytoin. Toxicol. Mech. Methods. 24: 353-361, 2014.

Patlar S., Boyali E., Baltaci A.K., Mogulkoc R., Gunay M. Elements in sera of elite taekwondo athletes: effects of vitamin E supplementation. Biol. Trace. Elem. Res. 139: 119-125, 2011.

Perucca P., Gilliam F.G. Adverse effects of antiepileptic drugs. Lancet Neurol. 11: 792-802, 2012.

Pillai R., Uyehara-Lock J.H., Bellinger F.P. Selenium and selenoprotein function in brain disorders. IUBMB. Life. 66: 229-239, 2014.

Prasad D.K., Shaheen U., Satyanarayana U., Surya Prabha T., Jyothy A., Munshi A. Association of serum trace elements and minerals with genetic generalized epilepsy and idiopathic intractable epilepsy. Neurochem. Res. 39: 2370-2376, 2014.

Putnam M.E. and Comben. N. Vitamin E. Vet. Rec. 121: 541-545, 1987.

Razazizan N., Mirmoeini M., Daeichin S., Ghadiri K. Comparison of 25-hydroxy vitamin D, calcium and alkaline phosphatase levels in epileptic and non-epileptic children. Acta. Neurol. Taiwan. 22: 112-126, 2013.

Saad K., Hammad E., Hassan A.F., Badry R. Trace element, oxidant, and antioxidant enzyme values in blood of children with refractory epilepsy. Int. J. Neurosci. 124: 181-186, 2014.

Saghazadeh A., Mahmoudi M., Meysamie A., Gharedaghi M., Zamponi G.W., Rezaei N. Possible role of trace elements in epilepsy and febrile seizures: a meta-analysis. Nutr. Rev. 73: 760-779, 2015.

Sen F.B., Ucar D., Ayyildiz M., Agar E. The Role of Cannabinoid cb1 Receptor in Proconvulsant Effect of Apelin-13 on Penicillin-induced Epileptiform Activity. Acta. Physiol. 709: 83-83, 2016.

Singh A., Papanicolaou D.A., Lawrence L.L., Howell E.A., Chrousos G.P., Deuster P.A. Neuroendocrine responses to running in women after zinc and vitamin E supplementation. Med. Sci. Sports. Exerc. 31; 536-542, 1993.

Ulas M. and Cay M. 17β-Estradiol and vitamin E modulates oxidative stress-induced kidney toxicity in diabetic ovariectomized rat. Biol. Trace. Elem. Res. 144: 821-831, 2011.

Ulatowski L. and Manor D. Vitamin E trafficking in neurologic health and disease. Annu. Rev. Nutr. 33: 87-103, 2013.

Wojciak R.W., Mojs E., Stanislawska-Kubiak M., Samborski W. The serum zinc, copper, iron, and chromium concentrations in epileptic children. Epilepsy Res. 104: 40-44, 2013.

Yorulmaz H., Seker F.B., Demir G., Yalcın I.E., Oztaş B. The effects of zinc treatment on theblood-brain barrier permeability and brain element levels during convulsions. Biol. Trace. Elem. Res. 151: 256-262, 2013.

Yuen A.W. and Sander J.W. Can magnesium supplementation reduce seizures in peoplewith epilepsy? A hypothesis. Epilepsy Res. 100: 152-156, 2012.



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